Treatment of myelomatosis--M.R.C. trial.

The zones can be measured by the fluorescence intensity emitted at an angle of 45'. The height and area of the peaks over the different zones corresponding to the fluorescence emission of that zone may be measured as relative percentages of the total fluorescence. The method uses unconcentrated early morning urine: 20 ILI are applied to paper and electrophoresed for four hours at 200 volts in Michaelis buffer (barbital/acetate), pH = 8.6, A = 0,075. Measurements were made on a Zeiss spectrophotometer PQ2 with the Sterzl additions of fluorescence equipment, scanner, and recorder. The irradiating light is passed through filter M 365, fluroescence being measured at 460 nm (maximum of fluorescence emission after excitation at 365 nm).